Ligation Calculator

This tool determines the mass of insert needed for various inserts:vector molar ratios within the range suitable for typical ligation reactions.

Enter the data of the targets you are interested in and receive sequencing calculations

Required insert DNA mass [g] = vector DNA mass [g] x {insert DNA length / vector DNA length} x desired insert to vector molar ratio

Notes: Desired ratio is one from set {1, 2, 3, 5 ,7}

Insert length should be smaller than vector length

First, the insert and vector DNA is digested using restriction endonucleases to create DNA fragments with sticky (or blunt) ends. Next, the desired DNA fragments are isolated and subsequently joined followed by treatment with DNA ligase. Finally, the resulting intact vector is transformed into competent E. coli cells, and transformants are identified through suitable genetic selection methods.

Want to know more about the step-by-step process of DNA ligation and its typical reaction? Learn more.

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How does the QIAGEN Ligation Calculator work?

The QIAGEN Ligation Calculator helps you determine the optimal amounts of DNA fragments and vector needed for a successful ligation reaction. By inputting the sizes and concentrations of your DNA fragments and vector, the calculator provides the precise volumes required for your ligation setup.

Can the ligation calculator be used for different types of DNA ligations?

Yes, the QIAGEN Ligation Calculator can be used for various types of DNA ligations, including sticky end ligations, blunt end ligations, and TA cloning. The calculator is designed to accommodate different ligation scenarios to help optimize your experimental outcomes.

How accurate are the results from the QIAGEN Ligation Calculator?

The results from the QIAGEN Ligation Calculator are based on standard biochemical principles and the input parameters you provide. While the calculator is designed to offer accurate and reliable recommendations, the actual success of your ligation reaction may depend on various factors, including the quality of your DNA and the efficiency of your ligase enzyme.