Transfection Cell Database - Detailed View


Cell Line: Primary monocyte

Cell Line Species/Tissue: Human / monocyte 
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
RPMI1640 
Percent Serum (%):
10 % 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
1x10e5 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
37.5 ng 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
no information 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
24 h 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 50 %  
 
Any modifications to the protocol?:
None  
Notes:
Analysis method: Real-Time RT-PCR  
References: