Transfection Cell Database - Detailed View


Cell Line: K562

Cell Line Species/Tissue: Human / Myeloid leukemia /  
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
RPMI 
Percent Serum (%):
10 % 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
12-well 
Number of Cells:
2x10e5 
Percent Confluence(%):
20% 
Amount of Nucleic Acid (µg):
20µM/3.3µl 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
18 µl 
Complex Incubation on Cells (hrs):
48 h, 72 h 
Analysis Performed Post-Transfection (hrs):
48 h, 72 h 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): ~85 %  
 
Any modifications to the protocol?:
None  
Notes:
Analysis QRT-PCR  
References: