| Cell Line Species/Tissue: |
Human
/
Cervix; epithelial; adenocarcinoma |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 % |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
4*10^5 |
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
1,5 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
12 µl |
Complex Incubation on Cells (hrs):
|
7 h |
Analysis Performed Post-Transfection (hrs):
|
48-72 h |
Transfection Efficiency (%):
|
50-60% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| I have used a PolyFect reagent as a control to other methods - standard calcium phosphate method and my own method to compare the transfection efficiency.For PolyFect I have got the best results. |
| References: |
| |