| Cell Line Species/Tissue: |
Human
/
Lung fibroblast |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
MEM with L-Glu; supplemented with Vitamins(1/100) and non essential aminoacids (1/100) |
Percent Serum (%):
|
20 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
30 mm wells (mostly in 6-well plates) |
Number of Cells:
|
225,000 per well |
Percent Confluence(%):
|
Approx. 60-70 % |
Amount of Nucleic Acid (µg):
|
2 µg per well |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
80 µl |
Complex Incubation on Cells (hrs):
|
24 h |
Analysis Performed Post-Transfection (hrs):
|
72 h |
Transfection Efficiency (%):
|
90 % |
| Knockdown Efficiency (%): |
80 % |
| |
| Any modifications to the protocol?:
|
| Adjusted the amount of HiPerFect Reagent |
| Notes: |
| |
| References: |
| |