| Cell Line Species/Tissue: |
Human
/
Lung epithelial |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI 1640 + Hepes |
Percent Serum (%):
|
No |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
1.000.000 cells/well |
Percent Confluence(%):
|
40-60% |
Amount of Nucleic Acid (µg):
|
20nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
12µl |
Complex Incubation on Cells (hrs):
|
24h |
Analysis Performed Post-Transfection (hrs):
|
24h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
80% |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| QIAGEN EGFR siRNA Alexaflour 488 labeled; RT-PCR Analsyis |
| References: |
| |