| Cell Line Species/Tissue: |
Human
/
Cervix; epithelial; adenocarcinoma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
|
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
see publication |
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
see publication |
Amount of Reagent (µl):
|
see publication |
Complex Incubation on Cells (hrs):
|
see publication |
Analysis Performed Post-Transfection (hrs):
|
see publication |
Transfection Efficiency (%):
|
see publication |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Triplicate, paired transfections were used in parallel normal-oxygen versus hypoxic incubations, dishes being exposed to hypoxia for 18-24h commencing 24h after transfection. |
| References: |
| Identification of an angiogenic mitogen selective for endocrine gland endothelium, Jennifer LeCouter et al., Nature/Vol 412/ 30 August 2001 |