| Cell Line Species/Tissue: |
human
/
T-cell leukemia |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI 1640, 2.5-10% serum |
Percent Serum (%):
|
2.5-10 |
Reporter System:
|
Luciferase,b-gal |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
4 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
8 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
10 RLU |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Electroporation gave 1 RLU |
| References: |
| |