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QIAamp 96 DNA QIAcube HT Kit (5)
Cat. No. / ID: 51331
For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers
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QIAamp 96 DNA QIAcube HT Kit
QIAcube HT Plasticware
This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
The QIAamp 96 DNA QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Simple and reliable automated processing for cost and time savings
- Standardized method suitable for a variety of sample types
- High yields even from specialized samples
- High-quality DNA that performs well in downstream analyses
- Optimized protocols for a range of starting materials
Product Details
The QIAamp 96 DNA QIAcube HT Kit enables simple, automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from blood, cells, and tissue samples. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis. This kit is designed to be used on QIAcube HT System.
Performance
The QIAamp 96 DNA QIAcube HT Kit provides fast and reliable automated purification of total DNA in 96-well format. Results are highly reproducible, with no significant well-to-well variation in DNA recovery (see figure Reproducible 96-well DNA purification).
Automated purification of total DNA from blood samples is quick, easy, and reliable with the QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed, and the resulting DNA is of the same high quality as from other QIAGEN purification solutions (see figures Agarose gel of purified genomic DNA and Analysis of genomic DNA from human blood).
QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit lets users increase sample purification throughput without having to compromise on quality or reliability. The procedure provides high yields of pure DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions (see figures High performance in downstream assays and Purification of genomic DNA from tissues).
Automated purification of total DNA from blood samples is quick, easy, and reliable with the QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed, and the resulting DNA is of the same high quality as from other QIAGEN purification solutions (see figures Agarose gel of purified genomic DNA and Analysis of genomic DNA from human blood).
QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit lets users increase sample purification throughput without having to compromise on quality or reliability. The procedure provides high yields of pure DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions (see figures High performance in downstream assays and Purification of genomic DNA from tissues).
See figures
Principle
The QIAamp 96 DNA QIAcube HT Kit enables automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen tissues, cells, and blood. Samples treated with common anticoagulants such as citrate, heparin, or EDTA are suitable for use. The procedure yields high-quality DNA that performs well in PCR and other enzymatic reactions.
The QIAamp 96 DNA QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
| Specification | Description |
|---|---|
| Number of samples | 24–96 samples (to be processed in increments of 8) |
| Sample input volume | Blood: up to 200 μl (for sample volumes less than 200 μl, add PBS) Cells: up to 5 x 106 cells Tissues: up to 20 mg tissue (10 mg for spleen, liver, or lung tissue) |
| Elution volume | 200 μl |
| Duration | 96 samples in approximately 96 minutes 24 samples in approximately 45 minutes |
Procedure
The QIAamp 96 DNA QIAcube HT procedure is fast and simple with the QIAcube HT instrument. Samples are first lysed using QIAGEN Proteinase K. Buffering conditions are adjusted to provide optimal DNA binding conditions and the lysate is loaded onto the QIAamp 96 plate. As vacuum is applied, DNA selectively binds to the QIAamp membrane, and contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in three efficient wash steps. Pure DNA is eluted under vacuum in a single step in 200 µl of Buffer AE.
Applications
The QIAamp 96 DNA QIAcube HT Kit provides high-quality DNA, ready to use in various downstream assays, including applications in:
Life science research Livestock breeding Pedigree genotyping Veterinary pathogen research Routine applied testing
Supporting data and figures
Agarose gel of purified genomic DNA.
Genomic DNA was purified from 50, 100, 150, and 200 µl human blood with QIAcube HT and the QIAamp 96 DNA QIAcube HT Kit. Purified DNA was eluted in 200 µl, and 7 µl of each eluate was analyzed on a 0.8% agarose gel in 1x TBE. M: Lambda HindIII marker.
Resources
Protocol Files (2)
Kit Handbooks (2)
Quick-Start Protocols (3)
Additional Resources (1)
Safety Data Sheets (1)
Technical Information (2)
Brochures & Guides (1)
Instrument User Manuals (1)
Certificates of Analysis (1)
FAQ
Can I run the QIAcube HT with the QIAxtractor software or vice versa?
For what batch size is the QIAcube HT Plasticware designed?
Can I upgrade a Corbett CAS-1820 to a QIAcube HT?
What file is required for reporting a QIAcube HT or QIAxtractor issue?
I am getting vacuum errors on my QIAcube HT. What should I do?
1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.
2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.
3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.
Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.
4. Make sure correct plates are used for the selected protocol.
5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.
If the issue persists, please contact QIAGEN Technical Service.
FAQ ID -9106
What do I do if the QIAcube HT or QIAxtractor software runs in virtual mode while the instrument is connected to the computer?