Cell Line Species/Tissue: |
Human
/
Neuroblastoma |
Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
|
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
12-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
60% |
Amount of Nucleic Acid (µg):
|
0.03µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
1,5µl |
Complex Incubation on Cells (hrs):
|
24h |
Analysis Performed Post-Transfection (hrs):
|
24h |
Transfection Efficiency (%):
|
80% |
Knockdown Efficiency (%): |
80% |
|
Any modifications to the protocol?:
|
|
Notes: |
|
References: |
|