| Cell Line Species/Tissue: |
Human
/
Liver |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM, antibiotic, fetal bovine serum |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
100-mm dish |
Number of Cells:
|
two million |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
10 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
50 |
Complex Incubation on Cells (hrs):
|
3 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
80% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |