Transfection Cell Database - Detailed View


Cell Line: HepG2

Cell Line Species/Tissue: Human / Hepatocarcinoma 
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
RPMI 
Percent Serum (%):
10% FCS 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well plate 
Number of Cells:
60.000 cells/well 
Percent Confluence(%):
~60-80%  
Amount of Nucleic Acid (µg):
1-10nM 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
3µl 
Complex Incubation on Cells (hrs):
48h 
Analysis Performed Post-Transfection (hrs):
48h 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 80-90%  
 
Any modifications to the protocol?:
None  
Notes:
qRT-PCR, QIAGEN MAPK1 siRNA, FastForward protocol works better  
References: