| Cell Line Species/Tissue: |
Rat
/
Neonatal cardiomyocytes |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10% FBS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
Cell preperation from 40-60 neonates |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
2 µl |
Amount of Reagent (µl):
|
4 µl |
Complex Incubation on Cells (hrs):
|
3 h |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
Not determined |
| Knockdown Efficiency (%): |
Reduction of Protein in Western Blot |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Li et al; Circulation Research Dec 12/26, 2003; Using DNA Microarray to identify SP1 as aTranscriptional Regulatory Element of Insulin-like growth factor 1 in Cardiac Muscle cells |