Transfection Cell Database - Detailed View


Cell Line: H4-II-E

Cell Line Species/Tissue: Rat / hepatoma 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM with antibiotics 
Percent Serum (%):
10% 
Reporter System:
b-gal 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
 
Number of Cells:
 
Percent Confluence(%):
~45 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
Beta-gal activity 1.588 
 
Any modifications to the protocol?:
 
Notes:
Beta-galactosidase activity was measured as conversion of CPRG to 564 nm absorbing product after 30 minute incubation  
References: