| Cell Line Species/Tissue: |
human
/
kidney epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
MEM |
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
40-50% |
Amount of Nucleic Acid (µg):
|
0.8µg |
Amount of Enhancer (µl):
|
6.4µl |
Amount of Reagent (µl):
|
10µl |
Complex Incubation on Cells (hrs):
|
4-8h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
30% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |