| Cell Line Species/Tissue: |
rat
/
heart muscle |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
96 mm well |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
10 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
20 |
Complex Incubation on Cells (hrs):
|
2-3 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
64% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Exp Clin Endocrinol Diabetes 107 (1999) 26-36; Efficiency posted is + or - 5%. |
| References: |
| |