| Cell Line Species/Tissue: |
human
/
histiocytic lymphoma, macrophage |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI, 10%FCS |
Percent Serum (%):
|
10 % |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
1-2x1000000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
2.5 µl |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
5.0 µl |
Complex Incubation on Cells (hrs):
|
24-30 hrs |
Analysis Performed Post-Transfection (hrs):
|
24-30 hrs |
Transfection Efficiency (%):
|
> 10% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |