| Cell Line Species/Tissue: |
rat
/
fibroblasts |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM low glucose+10%FCS |
Percent Serum (%):
|
10 |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
2x CsCl |
Plate Format:
|
12-well plate |
Number of Cells:
|
25K-50K |
Percent Confluence(%):
|
70 |
Amount of Nucleic Acid (µg):
|
0.5 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
7.5 |
Complex Incubation on Cells (hrs):
|
3 |
Analysis Performed Post-Transfection (hrs):
|
24-36 |
Transfection Efficiency (%):
|
50 |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |