| Cell Line Species/Tissue: |
Human
/
Carcinoma lung epithelial |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM + 10%FCS |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
60-mm dish |
Number of Cells:
|
1e6 |
Percent Confluence(%):
|
75 % |
Amount of Nucleic Acid (µg):
|
5 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
20 ml |
Complex Incubation on Cells (hrs):
|
3 h |
Analysis Performed Post-Transfection (hrs):
|
72 h |
Transfection Efficiency (%):
|
50 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |