Transfection Cell Database - Detailed View


Cell Line: MDCK

Cell Line Species/Tissue: canine / normal kidney, epithelial 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM + L-glutamine 
Percent Serum (%):
10 
Reporter System:
b-gal 
Plasmid Purification Method:
EndoFree Plasmid Kit 
Plate Format:
 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
24 
Transfection Efficiency (%):
20-25% 
 
Any modifications to the protocol?:
 
Notes:
Transfection efficiency determined by X-gal staining.  
References: