QIAgility Accessories

For rapid, high-precision automated PCR setup on the QIAgility

Products

QIAcuity Nanoplate Adapter (1)

Cat. No. / ID: 9027242

For holding 1x QIAcuity Nanoplate; for use with the QIAgility to automate nanoplate set up before digital PCR reaction on the QIAcuity Digital PCR System

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The QIAcuity Nanoplate Adapter is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Adapter, 72x0.1ml Strip Tubes

Cat. No. / ID: 9018917

For holding 72 x 0.1 ml Strip Tubes; tubes will fill a 72-Well Rotor (cat. no. 9018903)

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Adapter, Light Cycler 480 96-Well Plate

Cat. No. / ID: 9018937

For use with the Roche LightCycler 480; 96-well plate; wells hold 10–100 µl; plate has an orientation pin and a “front” indicator

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Adapter, 24x5ml Flat-Base Tubes

Cat. No. / ID: 9018934

For holding 24 x 5 ml Flat-Base Tubes

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Adapter, 33x1.8ml Screwtop Flat-Base Cryo Vials

Cat. No. / ID: 9018929

For holding 33 x 1.8 ml Nalgene or Corning Cryogenic Vials

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Adapter, 96x0.2ml PCR Tubes

Cat. No. / ID: 9018919

For holding 96 x 0.2 ml PCR tubes

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Adapter, 33x2ml Flat-Base Tubes

Cat. No. / ID: 9018930

For holding 33 x 2 ml Flat-Base Tubes

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Adapter, 33x1.5ml, Tapered-Base Tubes

Cat. No. / ID: 9018925

For holding 33 x 1.5 ml Tapered-Base Tubes

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Adapter, 36x0.2ml PCR Tubes

Cat. No. / ID: 9018918

For holding 36 x 0.2 ml PCR tubes; tubes will fill a 36-Well Rotor (cat. no. 9018907)

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Adapter, Flip-Cap 32x1.5ml Tapered-Base

Cat. No. / ID: 9018927

For holding 32 x 1.5 ml Tapered-Base Tubes; caps held vertically to enable access by the instrument; to be used in combination with the Flip-Cap Tube Lifter 32 x 1.5ml Tapered (cat. no. 9018950); a “virtual block” that holds 96 x 1.5 ml tubes can be configured using 3 adjacent adapters

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Adapter, 36x0.5ml PCR Tubes

Cat. No. / ID: 9018920

For holding 36 x 0.5 ml PCR Tubes; tubes are offset to make space for caps

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Adapter, Generic 384-Well Plate

Cat. No. / ID: 9019491

For use with generic 384-well plate

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Adapter, 48x0.5ml Tubes

Cat. No. / ID: 9018921

For holding 48 x 0.5 ml Tubes

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Cooling Block, Housing

Cat. No. / ID: 9020285

Cooling Block; Housing for use in combination with Cooling Block Insert (cat. no. 9020284)

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Flip-Cap Tube Lifter 32x1.5ml Tapered

Cat. No. / ID: 9018950

For quick and easy removal and resealing of tubes from an Adapter, Flip-Cap 32 x 1.5ml Tapered-Base (cat. no. 9018927)

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Cooling Block, Insert

Cat. No. / ID: 9020284

Cooling Block; for cooling SBS plates; add water to cooling block and allow to freeze; remove from freezer and stand for 10 minutes before use; fits in housing (cat. no. 9020285)

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Reagent Block, 16x0.2ml/1.5ml Tapered-Base Tubes

Cat. No. / ID: 9018952

Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 16 x 0.2 ml PCR tubes and 16 x 1.5 ml Tapered-Base Tubes

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Reagent Block, 24x1.5ml Tapered-Base Tubes

Cat. No. / ID: 9018955

Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 24 x 1.5 ml Tapered-Base Tubes

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Master Mix Block, 1x5ml/4x1.5ml Tubes

Cat. No. / ID: 9018958

Master Mix Block; intended to hold diluent and master mix on the instrument worktable; holds 1 x 5 ml Flat-Base Tube and 4 x 1.5 ml Tapered-Base Tubes

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Reagent Block, 16x0.2ml/2ml Flat-Base Tubes

Cat. No. / ID: 9018953

Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 16 x 0.2 ml PCR tubes and 16 x 2 ml Flat-Base Tubes

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Master Mix Block, 1x5ml/4x2ml Tubes

Cat. No. / ID: 9020130

Master Mix Block; intended to hold diluent and master mix on the instrument worktable; holds 1 x 5 ml Flat-Base Tube and 4 x 2 ml Flat-Base Tubes

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Reagent Block, 24x2ml Flat-Base Tubes

Cat. No. / ID: 9018956

Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 24 x 2 ml Flat-Base Tubes

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Master Mix Block, 5x5ml Tubes

Cat. No. / ID: 9018959

Master Mix Block; intended to hold diluent and master mix on the instrument worktable; holds 5 x 5 ml Flat-Base Tubes

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Reagent Block, 16x0.2ml/2ml Flat/1.5ml Tapered Tubes

Cat. No. / ID: 9018951

Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 16 x 200 µl PCR tubes, 8 x 2 ml Flat-Base Tubes, and 8 x 1.5 ml Tapered-Base Tubes

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Reagent Block, 8x1.5ml/2ml Flat/1.5ml Tapered Tubes

Cat. No. / ID: 9019499

Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 8 x 1.5 ml Tapered-Base Tubes, 8 x 2 ml Flat-Base Tubes, and 8 x 1.5 ml Tapered-Base Tubes

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Reagent Block, 17x5ml Flat-Base Tubes

Cat. No. / ID: 9018957

Reagent Block; intended to hold reagents and standards on the instrument worktable; holds 17 x 5 ml Flat-Base Tubes

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Tip Rack Holder for 96-Tip Rack

Cat. No. / ID: 9018949

Aluminum frame for disposable Tip Racks; includes spring-loaded locators for accurate alignment on the instrument worktable

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Adapter, Reagent Trough SBS Plate

Cat. No. / ID: 9018935

For holding combinations of 70 ml and 170 ml Reagent Troughs (cat. nos. 990554, 990556) in the SBS-standard plate position on the instrument worktable

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Loading Block, Rotor-Disc 100 Easylift Ejector

Cat. No. / ID: 9020286

Ejector to be used in combination with Loading Block, Rotor-Disc 100 Easylift (cat. no. 9020287)

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Loading Block, Rotor-Disc 100 Easylift

Cat. No. / ID: 9020287

Aluminium block for manual and automated reaction setup in Rotor-Disc 100 discs. The block covers 2 standard SBS positions in a vertical orientation on the QIAgility instrument worktable; ejection of the Rotor-Disc 100 is made convenient by using the Easylift ejector (cat. no. 9020286)

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Rotor-Disc 72 Loading Block

Cat. No. / ID: 9018910

Aluminium block for manual and automated reaction setup in Rotor-Disc 72 discs

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HEPA filter

Cat. No. / ID: 9019486

Replacement cartridge (one filter)

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Features

Elimination of manual pipetting steps
Automated PCR setup in all formats
Standardized results and increased productivity

Product Details

QIAgility accessories are for use with the QIAgility for rapid, high-precision PCR setup.

Resources

The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.

Addressing critical factors and new solutions

PCR 実験における重要項目と新技術

FAQ

No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.

FAQ ID -2117

Yes. The optimized master mix of the Rotor-Gene Multiplex PCR Kit ensures that all gene targets in a multiplex reaction are amplified with the same efficiency and sensitivity as in corresponding singleplex reactions, independent of starting copy numbers.

FAQ ID -2129

The Rotor-Gene Multiplex PCR Kit has been developed primarily for use with TaqMan® probes. Protocols for probes other than TaqMan® probes have not been tested.

See trademarks.

FAQ ID -2130

We strongly recommend use of the dedicated Rotor-Gene Q Accessories, e.g., PCR Tubes 0.2 ml or Strip Tubes and Caps 0.1 ml provided by QIAGEN. These tubes are designed to give low background and perfectly match the Rotor-Gene Q requirements.

FAQ ID -2085

The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.

FAQ ID -1519

There are several manufacturers of high-quality real-time cyclers. These include QIAGEN's Rotor-Gene Q, Applied Biosystems, BioRad, Stratagene, Eppendorf, Roche, TaKaRa, Fluidigm, and Cepheid. The important thing to keep in mind is that, once you select an instrument to use, you must use compatible Rotor-Gene Discs and tubes, 96 or 384 well plates, and qPCR master mixes that are optimized for use in that particular instrument. For example, QIAGEN's Rotor-Gene SYBR Green, Probe, and Multiplex real-time master mixes.

FAQ ID -2670

The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

FAQ ID -2116

Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.

FAQ ID -2119