QIAsymphony Virus/Bacteria Kits provide automated purification of viral nucleic acids and bacterial DNA from a broad range of sample types on the QIAsymphony SP. For example, nucleic acids can be purified from plasma, serum, CSF, respiratory samples, urine, urogenital swabs, and transport media. Kits are available in mini and midi format for sample volumes of 200 μl and up to 1000 μl. Optimized protocols provide high yields of pure nucleic acids, and flexible elution volumes allow nucleic acid concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.
Protocol | Kit | Sample material | Processed volume |
---|---|---|---|
Cellfree 200 | QIAsymphony Virus/Bacteria Mini Kit (192) | Plasma, serum, and CSF | 200 μl |
Cellfree 500 | QIAsymphony Virus/Bacteria Midi Kit (96) | Plasma, serum, and CSF | 500 μl |
Cellfree 1000 | QIAsymphony Virus/Bacteria Midi Kit (96) | Plasma, serum, and CSF | 1000 μl |
Complex 200 | QIAsymphony Virus/Bacteria Mini Kit (192) | Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) | 200 μl |
Complex 400 | QIAsymphony Virus/Bacteria Midi Kit (96) | Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) | 400 μl |
Complex 800 | QIAsymphony Virus/Bacteria Midi Kit (96) | Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media) | 800 μl |
QIAsymphony Virus/Bacteria Kits enable highly sensitive detection of a broad range of DNA and RNA viruses, as well as bacterial pathogens. Yields are linear, allowing accurate quantitative analysis for both low and high viral or bacterial titers. The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.
Features | Specifications |
---|---|
Applications | PCR, real-time PCR |
Elution volume | 60-110 µl |
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Purification of viral RNA and DNA and bacterial DNA |
Input volume | 800 µl (Pathogen Complex Protocol); 1000 µl (Virus Cellfree Protocol) |
Technology | Magnetic particles |
Sample types | Serum, plasma, CSF, cell-free body fluids, fresh or frozen material (body fluids, swabs, secretions, tissues, stool specimens) |