Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. RT
2 lncRNA PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments.
Flexible layout and controls
RT
2 lncRNA PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to monitor the expression of 84 lncRNA genes related to a disease state or pathway, plus 5 reference genes. Each RT
2 lncRNA PCR Array also includes control elements for:
- Data normalization
- Genomic DNA contamination detection
- RNA sample quality
- General PCR performance
Easy-to-use data analysis
Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔCq method with normalization of the raw data to either housekeeping genes.