Supplemental Microbial qPCR Mastermix (Fluorescein)

For real-time PCR applications

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Supplemental Microbial qPCR Mastermix (Fluorescein)

Cat. No. / ID: 330540

2 tubes of 1.35 ml each

The Supplemental Microbial qPCR Mastermix (Fluorescein) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

All-in-one mix for PCR using 5' hydrolysis probe-based detection
Yields specific products with minimal primer-dimers
Enables microbial profiling with instruments using fluorescein as a reference dye

Product Details

Supplemental Microbial qPCR Mastermix (Fluorescein) is highly suited for real-time PCR applications using 5' hydrolysis probe-based detection that uses fluorescein as a reference dye, including BioRad iCycler, MyiQ, and iQ5. This supplemental mastermix is for use in automated pipetting systems that require more mastermix in addition to the mastermix supplied with the Microbial DNA qPCR Arrays and Assay Kits. Supplemental Microbial qPCR Mastermix (Fluorescein) contains real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and fluorescein dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate 5' hydrolysis probe results by preventing the amplification of primer-dimers and other nonspecific products.

Principle

Supplemental Microbial qPCR Mastermix (Fluorescein) contains real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and fluorescein dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate 5' hydrolysis probe results by preventing the amplification of primer-dimers and other nonspecific products.

Applications

Supplemental Microbial qPCR Mastermix (Fluorescein) is highly suited for use in automated pipetting systems requiring additional mastermix in excess of the mastermix supplied with the Microbial DNA qPCR Arrays and Assay Kits.

Resources

For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes

FAQ

Store Microbial DNA qPCR Array plates at room temperature.
Store Microbial DNA qPCR Assays, Microbial DNA qPCR Assay Kits, and Microbial DNA qPCR Multi-Assay Kits at –20°C.
Store Microbial qPCR Mastermix at –20°C.
Store Microbial DNA Positive Control at –20°C.
Store Microbial DNA-Free Water at –20°C.

FAQ ID — 3394

The Microbial qPCR Mastermix (ROX) is suitable for use with the following real-time cyclers: all Applied Biosystems and Agilent (formerly Stratagene); BioRad Opticon, Opticon 2, and Chromo 4; Roche LightCycler 480; and Eppendorf Mastercycler ep realplex 2, 2S, 4, and 4S.

The Microbial qPCR Mastermix (Fluorescein) is suitable for use with the following real-time cyclers: BioRad iCycler, MyiQ, and iQ5.

FAQ ID — 3395