VeraSeq™ PCR Mix

Ultrathermostable mastermix for high-fidelity PCR

S_2962_GEN_generic
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VeraSeq PCR Mix (2x, 250 reactions)

Cat. No. / ID:   P7610L

250 reactions (evaluation pack) of 2x VeraSeq PCR Mix (1 x 6.25 mL)
The VeraSeq PCR Mix (2x, 250 reactions) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Certain applications in which this product can be used may be covered by patents issued and applicable in the United States and abroad. Purchase of this product does not include a license to perform any patented application; therefore, it is the sole responsibility of the users of the product to determine whether they may be required to engage a license agreement depending upon the particular application in which the product is used.
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM

Features

  • 2x master mix based on VeraSeq™ 2.0 (cat. no. P7511L)
  • 50x greater fidelity than Taq DNA Polymerase
  • Ultra-thermostable polymerase
  • Extends 1 kb in 15 seconds
  • Strong proofreading activity (3'→5' exonuclease activity) 

Product Details

VeraSeq™ PCR Mix is a premixed, ready-to-use 2x solution containing VeraSeq™ 2.0 High-Fidelity DNA Polymerase (P7511L), dNTPS, MgCl2 and reaction buffer at optimal concentrations to maximize the speed, accuracy and length of DNA synthesis. The formulation provides efficient, high-fidelity DNA amplification, cloning and synthetic biology applications.

Performance

Polymerase properties

  • Storage temperature: –25°C to –15°C
  • Extension rate: 15 second per kb at 72˚C 
  • Proofreading (3'→5' exo): Yes, strong 
  • Nick-translation (5'→3' exo): No 
  • Fidelity: >50x higher than Taq DNA Polymerase 
  • Strand displacement:  No 
  • Thermostability: Highly thermostable 
  • Able to extend an RNA primer: No
  • Extends from a nick: No 
  • Generate blunt end products: Yes 
  • Uracil read through: No
Test Specification
Functional Assay Amplification of 500 bp fragment from genomic DNA

Principle

Source of recombinant enzyme protein
The protein is produced by a recombinant E. coli strain carrying the engineered VeraSeq™ 2.0 gene.

Unit definition
One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form at 74°C in 30 minutes.

Procedure

Protocol
General precautions should be taken when setting up a PCR, including setting up the reaction on ice, adding master mix last, gently pipetting, thorough mixing and a quick centrifugation. The following procedure can be used as a guideline. Reactions may need to be optimized individually.

Reaction setup (for 50 µL)

Component Volume (µL) Final concentration
Sterile H2O 20, variable N/A
2x VeraSeq PCR Mix 25 1x
PCR Primer Cocktail 5 0.5 µM each
Library DNA* Variable N/A

* Total reaction volumes of library DNA and water should be adjusted to achieve a final reaction lolume of 50 µL. If the reaction volume needs to be >50 µL, the volume of the 2x Master Mix should be adjusted so that it constitutes 50% of the final reaction volume.

Step Temperature Time Cycles
Initial denaturation 98°C 30 seconds 1
Denaturation
Annealing
Extension
98°C
60°C
72°C
10 seconds
30 seconds
30 seconds
To be determined by user
Final extension 72°C
4°C
300 seconds
hold
1

* Cycling conditions may need to be optimized, depending on the amplicon of interest.
Number of cycles is dependent on the amount of input DNA and other specific sequence analysis requirements.

Quality control analysis


Functionality of 2x VeraSeq™ PCR Mix is assessed by its ability to amplify a 500 bp fragment from genomic DNA. Following PCR the 500 bp fragment was visualized by agarose-gel electrophoresis.

Contamination Tests

VeraSeq™ PCR Mix was tested prior to assembly and found to be free of contaminating endonucleases. Enzyme purity was >99% as determined by SDS-PAGE and negligible E. coli genomic DNA contamination was confirmed by qPCR. Specific activity was verified pre and post dilution.

Applications

This product is available for molecular biology applications such as:

  • High-fidelity DNA amplification
  • Cloning
  • Synthetic biology

Resources

Quick-Start Protocols (1)
Safety Data Sheets (1)
Certificates of Analysis (1)