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Cat. No. / ID: 9001301
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
The QIAsymphony SP is a versatile nucleic acid purification instrument providing high-quality nucleic acids compatible with with most common downstream assay technologies, such as real-time PCR, digital PCR, and next generation sequencing (NGS). The large range of a dedicated QIAsymphony kits enables optimized purification of genomic DNA, cell-free circulating DNA, RNA, bacterial and viral nucleic acids from a wide range of starting materials. The QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, which, in combination with the Rotor-Gene Q and QIAGEN real-time and end-point PCR kits, enables you to optimize your PCR workflow and maximize your efficiency.
The QIAsymphony SP can save you time by processing 1–96 samples — in batches of up to 24 — with sample volumes up to 10 ml. The dedicated range of optimized QIAsymphony kits and protocols guarantees best purification efficiency and nucleic acid quality and increases laboratory capabilities/flexibility with a single instrument. Customization service is available for adjusting standard protocols to your specific needs and specifications.
More information on QIAsymphony Kits and QIAsymphony Accessories>
Extending the QIAsymphony SP with the QIAsymphony AS results in a fully integrated system that can automate sample preparation and assay setup workflows without manual interference. Manual pipetting steps prone to human error are eliminated, making this system ideal for laboratories that perform complex assays on a day-to-day basis. The QIAsymphony AS provides active cooling of reagents, eluates and assays, enabling safe and reproducible assay setup. The QIAsymphony AS supports setup of multiple assays per run or sample. A customization service is available for in-house assays.
The QIAsymphony SP and the QIAsymphony SP/AS instruments deliver state-of-the-art process safety and standardization features. Tip guards minimize the risk of cross-contamination and built-in UV lamps provide effective worktable decontamination. Bar code reading of samples and reagents enables full tracking throughout the entire purification and assay setup procedure. Chronological electronic documentation of results is provided, including information about sample IDs, their positions, and the lot number and expiry date of sample preparation and PCR reagents. Multi-level user management provides a record of which user was responsible for sample processing. Electronic result files may be automatically printed via a networked printer, or downloaded using a USB stick for archiving purposes. The QIAlink software which is available separately (?) can handle communication with Laboratory Information Management Systems (LIMS).
The QIAsymphony Cabinet SP/AS (see figure " QIAsymphony Cabinet SP/AS") is specifically designed for correct positioning of the QIAsymphony SP/AS instruments. The QIAsymphony Cabinet SP/AS comprises two separate parts: one for the QIAsymphony SP and one for the QIAsymphony AS. If you have both QIAsymphony SP and QIAsymphony AS instruments, you need both cabinet, which are positioned side by side. If you have only a QIAsymphony SP instrument, only the QIAsymphony Cabinet SP is required. The QIAsymphony Cabinet SP and the QIAsymphony Cabinet AS each contain a waste compartment into which used tips from the worktable can be ejected.
In-vitro diagnostic use |
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Kit |
Cat. no. |
DNA extraction |
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QIAsymphony DSP DNA Mini Kit 937236 Purification of DNA from up to 200 µL human whole blood, 200 µL buffy coat, tissue (including FFPE tissue), bacterial and cell cultures, and purification of viral nucleic acids from blood |
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QIAsymphony DSP DNA Midi Kit 937255 Purification of DNA from up to 1000 µL human whole blood, 400 µL buffy coat, tissue (including FFPE tissue), bacterial and cell cultures, and purification of viral nucleic acids from blood |
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Pathogen viral DNA and RNA extraction |
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QIAsymphony DSP Virus/Pathogen Mini Kit 937036 Purification of viral nucleic acids from 200 µL of plasma, serum and CSF, and viral nucleic acids and bacterial DNA from respiratory and urogenital samples for in vitro diagnostic use |
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QIAsymphony DSP Virus/Pathogen Midi Kit 937055 Purification of viral nucleic acids from 1000 µL of plasma, serum and CSF, and viral nucleic acids and bacterial DNA from respiratory and urogenital samples for in vitro diagnostic use |
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Blood collection tubes and circulating DNA extraction | |
QIAsymphony PAXgene Blood ccfDNA Kit 768536 For purification of ccfDNA from plasma |
Research Use Only and Molecular Biology Applications |
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Kit |
Cat. no. |
DNA extraction | |
QIAsymphony DNA Mini Kit 931236 Purification of DNA from up to 200 µL human whole blood, 200 µL buffy coat, 50 mg tissue (including FFPE tissue), or 1 x 107 cells, and purification of viral nucleic acids from blood |
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QIAsymphony DNA Midi Kit 931255 Purification of DNA from up to 1000 µL human whole blood, 400 µL buffy coat, 50 mg tissue (including FFPE tissue), or 1 x 107 cells, and purification of viral nucleic acids from blood |
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QIAsymphony DNA Maxi Kit 937266 Purification of total DNA from up to 4 mL human whole blood samples |
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Pathogen viral DNA and RNA extraction |
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QIAsymphony Virus/Bacteria Mini Kit 931036 Simultaneous purification of viral DNA and RNA from 200 µL serum, plasma or cerebrospinal fluid and for viral DNA, viral RNA and bacterial DNA from respiratory and urogenital samples |
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QIAsymphony Virus/Bacteria Midi Kit 931055 Simultaneous purification of viral DNA and RNA from 1000 µL serum, plasma or cerebrospinal fluid and for viral DNA, viral RNA and bacterial DNA from respiratory and urogenital samples |
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Residual host cell DNA and viral nucleic acid extraction |
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QIAsymphony Certal Residual DNA Kit 931855 Purification of residual host cell DNA and viral nucleic acid from bioprocess purification buffer and cell culture supernatant samples |
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QIAsymphony Certal Vaccine NA Kit 931955 Purification of residual host cell DNA and viral nucleic acid from vaccine preparations |
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Microbial genomic DNA extraction |
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QIAsymphony mericon Bacteria Kit 931156 Purification of bacterial DNA from 500 μl enrichment culture |
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DNA extraction for various applications (microbiome, methylome, forensics) |
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QIAsymphony PowerFecal Pro DNA Kit 938036 Purification of microbial genomic DNA from stool and soil samples |
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QIAsymphony Bisulfite Kit 931106 For sample lysis and complete bisulfite conversion/cleanup of DNA using the QIAsymphony SP, optimized for downstream methylation analysis |
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QIAsymphony DNA Investigator Kit 931436 Purification of DNA from a variety of casework and reference samples for human ID applications |
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RNA extraction |
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QIAsymphony RNA Kit 931636 Purification of total RNA and miRNA from up to 50 mg tissue (including FFPE tissue) or 1 x 107 cells |
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Blood collection tubes and circulating DNA extraction |
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QIAsymphony PAXgene Blood ccfDNA Kit 768536 Purification of circulating cell-free DNA from plasma using the QIAsymphony SP instrument, to be used only in conjunction with PAXgene Blood ccfDNA Tubes |
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Blood collection tubes and RNA extraction |
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QIAsymphony PAXgene Blood RNA Kit 762635 Automated purification of intracellular RNA (including miRNAs) from stabilized blood |
The QIAsymphony SP is ready-to-run and requires only minimal installation. Laboratories already operating a QIAsymphony SP can easily upgrade their system onsite with a QIAsymphony AS module. The QIAsymphony AS directly interfaces with the QIAsymphony SP. To reduce manual handling steps and minimize the risk of sample contamination, samples processed on the QIAsymphony SP can be transferred automatically to the QIAsymphony AS (integrated operation). For extra flexibility, the QIAsymphony SP and AS can also be operated independently. Samples that have been processed earlier in the day, or that have been processed on a different QIAsymphony SP, may be manually transferred to the QIAsymphony AS (see figure " QIAsymphony workflows").
Sample preparation and assay setup runs are defined using the touchscreen. Simply load drawers with the required samples, reagents, and consumables. When each drawer is closed, a detailed inventory scan is performed. This ensures that the worktable is correctly set up for the selected protocols. After sample preparation, either remove purified nucleic acids from the eluate drawer or automatically transfer eluates to the QIAsymphony AS via the transfer module for assay setup. After assay setup, assays can be transferred directly to a real-time PCR cycler for analysis.
The sample input drawer accommodates a wide range of sample input formats to suit varying workflows and sample preparation requirements. Bar code-labeled primary and secondary sample tubes (diameter 10–16.9 mm), microplates, and blocks are supported.Continuous loading in batches of up to 24 samples plus internal controls, and the option to assign different protocols to each batch, delivers increased efficiency and walkaway time. The QIAsymphony AS enables setup of single or multiple PCRs per run or sample, splitting of eluates for panel testing, and active cooling of eluates, reagents, and PCRs. A choice of output adapters enables use of different real-time PCR cyclers (e.g., Rotor-Gene Q, 96-well cyclers, 32-capillary cyclers). To use the Rotor-Gene Q for subsequent detection, simply set up your PCRs in Strip Tubes or Rotor-Discs.
QIAsymphony Kits are safe and exceptionally easy to use. Ready-to-run, bar code-labeled reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid and saves you valuable time. Simply place up to 2 reagent cartridges in the QIAsymphony drawer. The reagent cartridges can be from the same or different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent cartridges are automatically opened by the instrument, and reagent volumes only for the selected number of samples are used, giving you complete cost control.
The QIAsymphony SP is an optimal nucleic acid purification system for most applications and workflows
As an integrated system in combination with the QIAsymphony AS, the QIAsymphony SP can automate workflows from sample to PCR setup ready for amplification.
QIAsymphony operating software guides the user step-by-step through setting up a sample preparation or assay setup run, with options to select and adapt standardized protocols. Required volumes of buffers, reagents, master mix, and controls are automatically calculated, and the user is informed of the number and type of consumables to be loaded. Transfer of sample loading information from the QIAsymphony SP to the QIAsymphony AS is convenient and reduces the number of steps required for assay definition.
The QIAsymphony SP/AS instruments provide standardized communication interfaces. Work lists and rack files may be imported to the instruments, enabling automatic run definition before samples are received in the laboratory. After assay setup, sample lists can be exported to selected real-time PCR cyclers. This bi-directional data exchange allows integration of QIAsymphony SP/AS instruments into laboratory information management systems (LIMS).
Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?
If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Features | Specifications |
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Power | 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages |
Input volume | QIAsymphony SP — minimum input volume: 200 µl (application dependent) |
Operating temperature | 15–32°C |
Pollution level | 2 |
Place of operation | For indoor use only |
Elution volume | QIAsymphony SP — 30–500 µl (application dependent) |
Kits or Application Packs designed for this instrument | QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits |
Overvoltage category | II |
Humidity | Relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C |
Instrument dimensions | QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm |
Processing | Automated |
Protocols/main application on this instrument | QIAsymphony SP — Purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials; QIAsymphony AS — PCR setup. |
Storage temperature | 5–40°C in manufacturer's package |
Technology | QIAsymphony SP — QIAGEN magnetic-particles chemistry |
Transportation temperature | –25 to 70°C in manufacturer's package |
Weight | QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg |
Altitude | Up to 2000 m (6500 ft) |