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QIAprep 96 Plus Miniprep Kit (4)
Cat. No. / ID: 27291
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well blocks.
ForFor instrument
QIAvac 96
BioRobot Universal
QIAprep 96 Plus Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Consistently high yields of up to 50 µg
- Pure preps suitable for a wide range of applications
- Vacuum, centrifugation, and automated processing options
Product Details
QIAprep 96 Plus Kits enable plasmid preparation in 96-well format. Samples can be processed using QIAvac 96 and/or a centrifuge or automated on the BioRobot Universal System. Up to 50 µg of high-quality plasmid DNA (endotoxin levels <10 EU/µg DNA) can be obtained from up to 5 ml of culture.
For optimal results it is recommended to use this product together with QIAvac 96.
Performance
High-purity preps and outstanding yields
QIAprep 96 Plus Kits provide a unique method for preparation of highly pure DNA (see figure " Superior plasmid DNA quality"). Yields of up to 50 µg can be consistently achieved using an optimized procedure. The unique binding buffer provided with the kits ensures very specific binding conditions (see figures " High yields of plasmid DNA" and " Superior performance compared to other suppliers").
See figures
Principle
The unique kit chemistry and design of the Plasmid Plus 96 plates allow high binding capacities. After neutralization, a TurboFilter 96 plate is used for lysate clearing. To optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate, a nonchaotropic binding buffer is added to the cleared lysate. The unique binding buffer ensures very specific binding conditions, resulting in DNA quality that is comparable to anion-exchange preps (see figure " Superior plasmid DNA quality"). Due to unique kit chemistry, up to 50 µg plasmid DNA per well can be obtained from a 5 ml E. coli culture (see figures " High yields of plasmid DNA" and " Superior performance compared to other suppliers").
See figures
Procedure
QIAprep 96 Plus Kits allow plasmid preparation in 96-well format. The QIAprep 96 Plus Miniprep protocol is based on a modified alkaline lysis procedure. Following lysate clearing, a simple bind-wash-elute procedure results in highly concentrated plasmid DNA, ready for direct use in subsequent applications (see flowchart " QIAprep 96 Plus Miniprep procedure").
Convenient and flexible processing
The QIAprep 96 Plus Miniprep Kit can be conveniently processed using the QIAvac 96 and/or a centrifuge, while preps can be automated on the BioRobot Universal System using the QIAprep 96 Plus BioRobot Kit. Optimized protocols ensure easy and straightforward plasmid DNA preparation in 96-well format.
See figures
Applications
Plasmid DNA purified using QIAprep 96 Plus Kits is highly suitable for a wide range of applications, including:
- Preparation of short hairpin vectors (sh-vectors)
- Enzymatic modifications
- Cloning
- Restriction digestion
- Sequencing
Supporting data and figures
High yields of plasmid DNA.
E. coli DH5 a strains carrying pCMVβ were grown in 5 ml LB medium. Plasmid DNA was isolated manually using the QIAvac 96 and either the QIAprep 96 Plus Miniprep Kit or the QIAprep 96 Turbo Miniprep Kit or automated on the BioRobot Universal System using either the QIAprep 96 Plus BioRobot Kit or the QIAprep 96 Turbo BioRobot Kit. DNA was quantified using absorbance measurement and verified by agarose gel analysis. Higher yields were achieved with QIAprep 96 Plus Miniprep and BioRobot Kits.
Specifications
| Features | Specifications |
|---|---|
| Applications | Preparation of short hairpin vectors (sh-vectors), enzymatic modifications, cloning, restriction digestion, sequencing, etc |
| Technology | Nonchaotropic binding chemistry |
| Time per run or prep per run | 40 minutes |
| Yield | 15–50 µg |
| Culture volume/starting material | 1.25–5 ml culture volume |
| Samples per run (throughput) | 48–96 samples per run |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing | QIAvac 96, centrifuge, automated on BioRobot Universal or Tecan Resolvex A200 |
Resources
Kit Handbooks (2)
Quick-Start Protocols (1)
Application Notes (1)
Safety Data Sheets (1)
Brochures & Guides (1)
Certificates of Analysis (1)
FAQ
For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?