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QIAamp 96 DNA Swab BioRobot Kit (12)
Cat. No. / ID: 965842
For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
The QIAamp 96 DNA Swab BioRobot Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Rapid purification of high-quality DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Removal of contaminants and inhibitors
Product Details
The QIAamp 96 DNA Swab BioRobot Kit provides automated DNA purification from up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. Up to 192 samples are processed in less than 2.5 hours. The procedure is optimized for use with air-dried swabs with plastic shafts and cotton or DACRON tips, although other swab types can be used.
Performance
Proven QIAamp 96 technology provides well-to-well uniformity in DNA recovery and purity with no cross-contamination between samples (see figure " Cross-contamination test"). The effective removal of enzyme inhibitors ensures reliable performance in downstream applications such as TaqMan®, LightCycler, and iCycler analyses (see figure " Reliable performance").
DNA isolated with the QIAamp 96 DNA Swab BioRobot Kit is ready to use in a wide range of applications in molecular diagnostics and forensic science:
- Genetic disease testing, such as hemochromatosis or Factor V Leiden
- Identity testing, such as HLA typing
- Paternity testing (see figures " Multiplex STR analysis" and " GeneScan analysis")
See figures
Principle
No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in four efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields DNA from swabs ready to use in PCR and blotting procedures.
Procedure
The QIAamp 96 DNA Swab BioRobot Kit simplifies isolation of DNA from swabs with a fast automated 96-well–plate procedure (see flowchart " QIAamp 96 DNA Swab BioRobot procedure"). The procedure is optimized for use with air-dried swabs with plastic shafts and cotton or DACRON tips, although other swab types can be used. The typical yield for the QIAamp 96 DNA Swab BioRobot Kit is 1–2 µg per swab (buccal swabs), with an elution volume of 150 µl.
QIAamp sample preparation technology is fully licensed.
See figures
Applications
The QIAamp 96 DNA Swab BioRobot Kit provides QIAamp technology in a convenient 96-well format for high-throughput purification needs. The DNA from up to 96 swab samples is purified in under 2 hours. Sample types include:
- Buccal swabs
- Pharyngeal swabs
- Eye swabs
Supporting data and figures
High sensitivity in PCR and RT-PCR using QIAamp MinElute Virus Kits.
The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. 95% probit values for 40 samples were 27.25 IU/ml for the spin procedure and 9.30 IU/ml for the vacuum procedure.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, blotting |
| Yield | 1–2 µg per swab |
| Format | 96-well plate |
| For automated processing | BioRobot Universal System, BioRobot Genotyping, BioRobot 9604 |
| Sample amount | 1 swab per well |
| Processing | Automated |
| Elution volume | 150 µl |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA |
| Main sample type | Swabs |
| Technology | Silica technology |
| Time per run or per prep | <2 hours |
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
Publications
Estrogen sulfation genes, hormone replacement therapy, and endometrial cancer risk.
J Natl Cancer Inst; 2006; 98 (18):1311-20 2006 Sep 20 PMID:16985250
Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.
Leg Med (Tokyo); 2003; 5 Suppl 1 :S145-9 2003 Mar PMID:12935575
FAQ
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
How do I safely inactivate biohazardous flow-through material?