BioSprint DNA Plant Kits

For rapid and economical automated purification of total DNA from plant tissue

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

BioSprint 96 DNA Plant Kit (576)

Cat. No. / ID: 941557

For 576 preps: Large 96-Rod Covers, 96-Well Microplates MP, S Blocks, MagAttract Suspension G, buffers and reagents

Preparations

576

1536

Inquire

BioSprint DNA Plant Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

High-throughput isolation of DNA
Maximal flexibility to accommodate challenging sample material
Complete removal of contamination and inhibitors
No organic extraction or alcohol precipitation

Product Details

BioSprint DNA Plant Kits provide all reagents and plasticware necessary for automated purification of total DNA from plant tissue using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.

Performance

DNA can be successfully purified from a variety of plant species and material (see tables “DNA purified from a wide range of plant species using the BioSprint 96”).

DNA purified from a wide range of plant species using the BioSprint 96

Sample type	Amount	Yield (µg)
Arabidopsis, fresh leaves	50–120 mg	13–41
Cotton, fresh leaves	50 mg	10–26
Maize, fresh leaves	50 mg	10–41
Pine, fresh needles	100–190 mg	5–18
Rose, fresh new leaves	100–140 mg	40–100
Rose, fresh old leaves	100–140 mg	11–51
Soybean, fresh leaves	50 mg	39–80
Spruce, fresh needles	130–160 mg	5–13
Wheat, fresh leaves	50 mg	8–49
Maize, lyophilized leaves	5–10 mg	10–15.5
Rapeseed, lyophilized leaves	5–10 mg	13.5–23
Sunflower, lyophilized leaves	5–10 mg	57–93
Maize, dried seed powder	20–55 mg	9.8–33.5
Apple	1–6 seeds	5–30
French bean	1 bean	13–55
Grape	3 seeds	18.2–18.8
Linen	1–10 seeds	1.5–38
Lupine	2–7 seeds	18–97
Orange	1 seed	1–35
Rapeseed	1–4 seeds	8.5–47.5
Soybean	1 seed	80–105
Swedish turnip	5–50 seeds	21.5–52
Tomato	15–30 seeds	12–16
Turnip radish	4–18 seeds	11–41
Wheat	3–4 seeds	85–122

Purified DNA is up to 40 kb in size (see figure " Reproducible purification of high-quality DNA"). The DNA is ready to use in a range of downstream applications, including PCR and real-time PCR, genotyping and microsatellite analysis (see figures " High PCR performance of DNA purified using the BioSprint 15 DNA Plant Kit” and " High PCR performance of purified DNA”).

See figures

Reproducible purification of high-quality DNA.

High PCR performance of DNA purified using the BioSprint 15 DNA Plant Kit.

Purification of high-quality genomic DNA from mouse tail.

Principle

BioSprint DNA Plant Kits are used with the BioSprint 15 and BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from plant tissue. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure " Efficient processing of magnetic particles"). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.

See figures

Efficient processing of magnetic particles.

Procedure

BioSprint DNA Plant Kits enable purification of total DNA from a wide variety of plant species, including difficult plant material. Fresh, frozen or lyophilized starting material is mechanically disrupted to give a fine powder using a homogenizer such as the TissueLyser LT or TissueLyser II. The powder is resuspended in lysis buffer, thoroughly mixed and then sedimented by a short centrifugation step. Cleared plant lysates are transferred to a 5-well strip or S-Block and loaded into the instrument, which then performs all steps until pure DNA is obtained (see flowchart " BioSprint DNA Plant procedure"). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables.

See figures

BioSprint DNA Plant procedure.

Applications

BioSprint DNA Plant Kits purify DNA for use in a range of downstream applications, including:

PCR
Real-time PCR
Genotyping
Microsatellite analysis

Comparison of BioSprint DNA Plant Kits

Features	BioSprint 15 DNA Plant Kit	BioSprint 96 DNA Plant Kit
Compatible instrument	BioSprint 15	BioSprint 96 Thermo KingFisher Flex
Applications	PCR, real-time PCR, genotyping	PCR, real-time PCR, genotyping
Elution volume	200 µl	200 µl
For automated processing	BioSprint 15 workstation	BioSprint 96 workstation
Format	5-tube strip, S-Block	5-tube strip, S-Block
Main sample type	Plant samples	Plant samples
Processing	Automated	Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	DNA	DNA
Sample amount	10–100 mg	10–100 mg
Technology	Magnetic particles	Magnetic particles
Time per run or per prep	18 minutes	15 minutes
Yield	5–50 µg	5–20 µg

Supporting data and figures

Reproducible purification of high-quality DNA.

Genomic DNA was purified from 50 mg samples of fresh leaf tissue from [A] arabidopsis, [B] maize, and [C] rose. Purification was performed using the BioSprint 96 DNA Plant Kit and BioSprint 96 DNA Plant protocol. Purified DNA was eluted in 200 µl Buffer AE. Eluates (5 µl) were visualized on a 0.8% agarose gel. M: markers (1 kb ladder).

Resources

FAQ

No, the BioSprint Instruments should not be operated in a cool room. Optimal operation temperature for BioSprint is 10-40°C. Operation at 4°C may harm the instruments.

FAQ ID -938

Yes. We have shown that DNA purified with the BioSprint DNA Kits (e.g., the BioSprint 15 DNA Blood Kit, BioSprint 15 DNA Plant Kit, BioSprint 96 DNA Blood Kit and BioSprint 96 DNA Plant Kit) is stable for at least 2 years when being stored at either 2-8°C or -20°C. Our data indicate however that DNA stability is dependent upon storage conditions. For additional information see also FAQ 518.

FAQ ID -1318

Yes, DNA can be isolated from lyophilized plant material on the BioSprint 96 instruments using the BioSprint 96 DNA Plant Kit. We recommend starting with no more than 10 or 30 mg of lyophilized plant, respectively. Optimal starting amounts depend on plant type and need to be determined empirically.

FAQ ID -772

Typically, DNA yields obtained on the BioSprint 96 range from 5 to 20 ug of DNA from 10 to 100 mg of fresh/frozen plant material using the BioSprint 96 DNA Plant Kit. Yield depends on sample type, sample collection method, and method of tissue disruption.

FAQ ID -771

Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample
Mix, and store at –20°C for at least 1 h to precipitate the DNA
Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15–20 min
Pour off the ethanol and wash the pellet twice with room-temperature 70% ethanol
Allow the DNA pellet to air-dry
resuspend the DNA in a suitable volume of sterile TE buffer or distilled water

FAQ ID -305

The BioSprint 96 instrument has a magnetic-particle collecting efficiency above 95%. However, to avoid particle carryover from the eluate plate to subsequent applications, you can use a plate magnet while pipetting.

FAQ ID -2073

Measure the absorbance of the eluate obtained on the BioSprint 96 instruments at 260 nm, 280 nm and 320 nm. Carryover of magnetic particles in the eluates may affect the A260 and A280 readings, but should not affect the performance of the DNA in downstream applications. Subtract the absorbance reading obtained at 320 nm from the readings obtained at 260 nm and 280 nm to correct for the presence of residual magnetic particles.

FAQ ID -773

Bisulfite conversion of unmethylated cytosines into uracils with the EpiTect Bisulfite Kit works on DNA irrespective of the source organism. The DNA template needs to be of high purity for efficient conversion. We recommend to use genomic DNA extracted with our DNA isolation kits for clinical or animal and plant samples as a template for the EpiTect Bisulfite Kit.

FAQ ID -1209

Yes, the RLT lysate generated during the BioSprint DNA Plant protocol can be stored frozen. Redissolve any precipitates that may have formed during storage by gentle heating and continue with the isolation procedure.

FAQ ID -1248