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artus Parvo B19 LC PCR Kit (96) RUO
Cat. No. / ID: 4504005
For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, Mg Solution, 5 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- High reliability using the internal control
- Sensitive detection of as few as 0.2 IU/µl (ABI PRISM 7000)
- Accurate quantitation using the 5 standards supplied
Product Details
artus Parvo B19 PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for reliable parvovirus B19 DNA detection and quantitation.
Performance
To ensure sensitivity, artus Parvo B19 PCR Kits have been optimized to detect low numbers of parvovirus B19 DNA. The analytical sensitivity of the artus Parvo B19 TM PCR Kit is 0.2 IU/µl in the PCR on the ABI PRISM 7000 SDS.
| Kit | artus Parvo B19 LC PCR Kit | artus Parvo B19 TM PCR Kit |
|---|---|---|
| Sample type | Serum or plasma | Serum or plasma |
| Analytical sensitivity | 125 IU/ml (plasma, QIAamp UltraSens Virus Kit) | 0.2 IU/µl in the PCR |
Principle
artus Parvo B19 PCR Kits are based on the amplification and simultaneous detection of a specific region of the parvovirus B19 genome using real-time PCR. The kits provide high levels of specificity and sensitivity.
Each artus Parvo B19 PCR Kit provides 5 quantitation standards (see figure " Reliable quantitation of parvovirus B19 load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical parvovirus B19 PCR is not reduced.
| Kit | artus Parvo B19 LC PCR Kit | artus Parvo B19 TM PCR Kit |
|---|---|---|
| Sample types | Serum or plasma | Serum or plasma |
| Amplicon | 259 bp region of the parvovirus B19 genome | 76 bp region of the parvovirus B19 genome |
See figures
Procedure
artus Parvo B19 PCR Kits provide all necessary reagents optimized for reliable parvovirus B19 DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix (and Mg solution, LC kit only), and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
For DNA purification, we recommend using the QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit.
Applications
artus Parvo B19 PCR Kits enable the rapid and sensitive detection of parvovirus B19 DNA from serum and plasma with accurate quantification. The kits are available for use on the LightCycler 1.1/1.2/1.5 Instruments or the ABI PRISM 7000, 7700, 7900HT SDS.
Supporting data and figures
Reliable quantitation of parvovirus B19 load.
Detection of the quantitation standards (Parvo B19 RG QS 1–5) on the Rotor-Gene using the artus Parvo B19 RG PCR Kit. NTC: no template control.
Resources
Kit Handbooks (4)
Safety Data Sheets (1)
Certificates of Analysis (1)