To ensure sensitivity, artus HSV-1/2 Kits have been optimized to detect low copy numbers of HSV-1/2 DNA. The analytical sensitivity of the artus HSV-1/2 QS-RGQ Kit is 57.3 copies/ml for HSV-1 and 65.7 copies/ml for HSV-2 in consideration of the purification and assay setup using the QIAsymphony RGQ (see figure " Sensitive detection of HSV-1 DNA").
Kit | artus HSV-1/2 RG PCR Kit | artus HSV-1/2 QS-RGQ Kit |
---|---|---|
Sample type | Cerebral spinal fluid (CSF) | Cerebral spinal fluid (CSF) |
Analytical sensitivity | 0.12 copies/μl HSV-1, 0.16 copies/μl HSV-2 in the PCR | 57.3 copies/ml HSV-1, 65.7 copies/ml HSV-2 in consideration of the purification |
Specificity | Tested with HSV-1 (HF, KOS, MacIntyre strains) and HSV-2 (HG-52, G, and MS strains) | Tested with HSV-1 (HF, KOS, MacIntyre strains) and HSV-2 (HG-52, G, and MS strains) |
artus HSV-1/2 PCR Kits are based on the amplification and simultaneous detection of a specific region of the HSV-1 and HSV-2 genome using real-time PCR. The kits provide high levels of specificity and sensitivity (see figure " Sensitive detection of HSV-1 DNA").
The kits provide positive controls for HSV-1 and HSV-2 (see figures " Detection of the HSV-1 positive control" and "Detection of the HSV-2 positive control"). In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR.
Kit | artus HSV-1/2 RG PCR Kit and artus HSV-1/2 QS-RGQ Kit |
---|---|
Sample type | Cerebral spinal fluid (CSF) |
Amplicon | 154 bp region of the UL5 gene |
artus HSV-1/2 PCR Kits provide all necessary reagents optimized for reliable HSV-1/2 DNA detection and differentiation. Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
The temperature profiles of artus HSV-1/2 LC PCR, RG PCR, and QS-RGQ Kits correspond to the profiles of the artus LC PCR, RG PCR, and QS-RGQ Kits for CMV, EBV, VZV, and (RG and QS-RGQ kits only) BK virus. Therefore, these PCR assays can be performed and analyzed in a single run.
The QIAsymphony RGQ workflow solution for HSV-1/2 detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus HSV-1/2 QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete workflow (see figure " Integrated QIAsymphony RGQ system for HSV-1/2 detection").
For DNA purification, we recommend using the EZ1 DSP Virus Kit for automated viral nucleic acid purification.
artus HSV-1/2 PCR Kits enable rapid and sensitive detection of HSV-1/2 DNA from EDTA plasma. The kits enable discrimination of HSV-1 and HSV-2 using genotype specific probes.
The artus HSV-1/2 QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from sample to HSV DNA detection and discrimination of HSV-1 and HSV-2.