ssDNA Mass to Moles Conventer

This tool converts the mass of ssDNA to moles of ssDNA and moles of ssDNA to the mass of ssDNA.

Enter the data of the targets you are interested in and receive sequencing calculations

Mass to Moles Converter

moles of ssDNA [mol] = ssDNA mass [g] / DNA molecular weight [g/mol]
moles of ssDNA ends [mol] = moles of ssDNA [mol]

DNA copy number [molecules] = moles of ssDNA [mol] x 6.022e23 [molecules/mol]
DNA molecular weight [g/mol] = (DNA length [nt] x 308.97 [g/mol//nt]) + 18.02 [g/mol]

Note: 308.97 [g/mol] per nt is average where sequence not given or if sequence is given sum of A: 313.23 [g/mol]; T: 304.21 [g/mol]; C: 289.20 [g/mol];G: 329.23 [g/mol]

Moles to Mass Converter

ssDNA mass [g] = ssDNA moles [mol] x DNA molecular weight [g/mol]
DNA molecular weight [g/mol] = (DNA length [nt] x 308.97 [g/mol//nt]) + 18.02 [g/mol]

Note: 308.97 [g/mol] per nt is average where sequence not given or if sequence is given sum of A: 313.23 [g/mol] ; T: 304.21 [g/mol]; C: 289.20 [g/mol]; G: 329.23 [g/mol]

The ssDNA Mass Moles Converter is commonly used in molecular biology applications that involve quantifying single-stranded DNA (ssDNA) samples based on their mass and converting it to moles. This conversion is essential for various experimental procedures, such as oligonucleotide synthesis, ssDNA quantification and labeling reactions.

Learn more about these specific applications where the ssDNA Mass Moles Converter might be used:

1. Oligonucleotide synthesis: Oligonucleotides are short sequences of ssDNA that are synthesized for PCR primers, gene synthesis or molecular probes. The converter aids researchers in determining the amount of synthesized ssDNA in moles based on its mass, ensuring accurate calculations for the subsequent experimental steps.
2. ssDNA quantification: Similar to dsDNA quantification, the converter assists in identifying the concentration of ssDNA samples. This is essential for many applications, including hybridization experiments, ligations and molecular biology assays that require precise knowledge of the amount of ssDNA present.
3. Labeling reactions: Many labeling reactions, such as fluorescent labeling or radioisotope labeling, require accurately determining the amount of ssDNA used as a starting material. The converter helps in converting the mass of ssDNA into moles, enabling researchers to calculate the appropriate amount of labeling reagents needed for efficient labeling reactions.
4. Nucleic acid hybridization: Hybridization experiments, such as Southern blotting or in situ hybridization, often require quantification of ssDNA probes. The converter assists in converting the mass of ssDNA into moles, allowing accurate determination of probe concentrations for optimal hybridization conditions.

In summary, the ssDNA Mass Moles Converter is a valuable tool for converting ssDNA mass measurements to moles, facilitating accurate quantification and precise experimental design in various applications involving single-stranded DNA. Looking for enzymes to integrate into your workflows and experiments? Check out our broad spectrum portfolio.

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What is the ssDNA Mass to Moles Converter used for?

The ssDNA Mass to Moles Converter is designed to help you convert the mass of single-stranded DNA (ssDNA) into moles and vice versa. This conversion is crucial for accurately preparing ssDNA solutions for various molecular biology experiments

What information do I need to use the ssDNA Mass to Moles Converter?

To use the ssDNA Mass to Moles Converter, you need to know the length of your ssDNA in nucleotides and either the mass of the ssDNA in nanograms (ng) or the number of moles. This information allows the tool to perform the necessary conversions accurately.

How does the ssDNA Mass to Moles Converter perform the conversion?

The converter uses the average molecular weight of a nucleotide in single-stranded DNA, which is approximately 330 Daltons. By using this molecular weight, the tool converts the input mass of ssDNA into moles or the input moles into mass.