dsDNA Mass to Moles Conventer

This tool converts the mass of dsDNA to moles of dsDNA and moles of dsDNA to the mass of dsDNA.

Enter the data of the targets you are interested in and receive sequencing calculations

Mass to Moles Converter

moles of dsDNA [mol] = dsDNA mass [g] / DNA molecular weight [g/mol]
moles of dsDNA ends [mol] = moles of dsDNA [mol] x 2

DNA copy number [molecules] = moles of dsDNA [mol] x 6.022e23 [molecules/mol]
DNA molecular weight [g/mol] = (DNA length [bp] x 617.96 [g/mol per bp]) + 36.04 [g/mol]

Note: 617.96[g/mol] per bp is average molecule weight where sequence not given or if sequence is given sum of A: 617.44 [g/mol], T: 617.44 [g/mol], C: 618.43 [g/mol], G: 618.43 [g/mol]

Moles to Mass Converter

dsDNA mass [g] = DNA moles [mol] xDNA molecular weight [g/mol]
DNA molecular weight [g/mol] = (DNA length [bp] x 617.96 [g/mol per bp]) + 36.04 [g/mol]

Note: 617.96[g/mol] per bp is average molecule weight where sequence not given or if sequence is given sum of A: 617.44 [g/mol]; T: 617.44 [g/mol]; C: 618.43 [g/mol]; G: 618.43 [g/mol]

This converter is highly used in molecular biology applications involving quantifying DNA samples based on their mass and converting them to moles. This conversion is vital for various experimental procedures, such as PCR (polymerase chain reaction), DNA sequencing, cloning and gene expression analysis.

Here are some specific applications where you may use the dsDNA Mass Moles Converter:

1. DNA quantification: Determining the DNA concentration in a sample is crucial for many experiments. The converter helps researchers calculate the amount of DNA in moles based on the mass of dsDNA.
2. PCR and qPCR: Polymerase chain reaction (PCR) is used to amplify specific DNA sequences, while quantitative PCR (qPCR) allows for quantifying DNA or RNA targets. The converter can accurately determine the amount of DNA to be used as a starting template or in calculating standard curves for absolute quantification.
3. DNA sequencing: Prior to DNA sequencing, the concentration and purity of the DNA sample need to be determined. The converter assists in converting the mass of dsDNA into moles, which is necessary for accurate quantification and subsequent library preparation for sequencing.
4. Cloning experiments: The converter helps researchers determine the DNA required for specific cloning reactions in molecular cloning. This ensures the appropriate stoichiometry of DNA fragments, such as vectors and inserts, for successful cloning.
5. Gene expression analysis: Techniques like quantitative reverse transcription PCR (qRT-PCR) and RNA-seq rely on accurate quantification of DNA or RNA. The converter aids in determining the amount of starting material required for such analyses.

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What is the purpose of the dsDNA Mass to Moles Converter?

The dsDNA Mass to Moles Converter is a tool designed to help you convert the mass of double-stranded DNA (dsDNA) into moles and vice versa. This conversion is essential for accurately preparing DNA solutions for various molecular biology applications.

What information do I need to use the dsDNA Mass to Moles Converter?

To use the dsDNA Mass to Moles Converter, you need to know the length of your DNA in base pairs (bp) and either the mass of the DNA in nanograms (ng) or the number of moles. With this information, the calculator can perform the necessary conversions.

How does the dsDNA Mass to Moles Converter calculate the conversion?

The converter uses the average molecular weight of a base pair in double-stranded DNA, which is approximately 650 Daltons. By using this molecular weight, the tool converts the input mass of DNA into moles or the input moles into mass.