Complete genomic and transcriptome coverage, with low experimental variability
The REPLI-g Cell WGA & WTA Kit contains the novel REPLI-g SensiPhi DNA Polymerase, as well as an optimized set of buffers and reagents for parallel whole genome amplification (WGA) and whole transcriptome amplification (WTA) from 25–1000 cells, or equivalently small samples. Following efficient cell lysis, (and complete removal of genomic DNA followed by sensitive reverse transcription for the WTA reaction), the kit utilizes Multiple Displacement Amplification (MDA) technology for complete and unbiased amplification of gDNA and cDNA (see figure
Multiple Displacement Amplification (MDA) technology). cDNA amplified using the REPLI-g Cell WGA & WTA Kit demonstrates a high degree of reproducibility from experiment to experiment, with minimal “technical noise”, ensuring highly reliable results (see figure
High level of experimental reproducibility and minimized technical noise). The amplified gDNA and cDNA can be easily used in a variety of downstream applications (see figure
REPLI-g amplified cDNA and gDNA perform like gDNA in downstream experiments).
Ideal for analyzing the genomic and transcriptomic profiles of limited samples
The REPLI-g Cell WGA & WTA Kit provides highly uniform, parallel amplification across the entire genome and transcriptome, with negligible sequence bias. Even from samples as low as 25 cells, the kit ensures reliable whole genome and transcriptome amplification, making it highly suited for analyzing the direct consequences of genotypic changes on transcriptomes (see figure
Highly suited for simultaneous genomic and transcriptomic analysis from the same limited sample).
For use in a wide variety of research areas
The REPLI-g Cell WGA & WTA Kit efficiently amplifies gDNA and cDNA directly from cells and can be used with various samples that are often analyzed in clinical research, including tumor cells, stem cells, sorted cells, or other small samples (see table).
Wide range of sample material and research areas Sample material (cells/total RNA) | Research area |
Cancer | Somatic genetic variant analysis |
Tumor progression |
Tumor stem cells/evolution |
Tumor heterogeneity |
Somatic genetic variant analysis |
Human/animal | Biomarker research (SNPs, mutations, CNVs) |
Mosaicism studies |
Stem cell research |
Genetic predisposition studies |