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Isolation of plasmid DNA from Corynebacterium glutamicum using the QIAGEN® Plasmid Mini Kit - (EN)
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The procedure has been used successfully for isolation of different medium-copy-number plasmids carrying pHM1519 or pBL1 origins of replication from
Corynebacterium glutamicum
ATCC 13032. Yield of plasmid DNA was typically 0.4-1.5 µg per ml LB culture, although yield was dependent on the vector, the insert, and the size of the plasmid.
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